Composite

Part:BBa_K287005:Design

Designed by: Carlos Andres Sanchez   Group: iGEM09_IPOC1-Colombia   (2009-10-21)


Double Channel


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 214
    Illegal SpeI site found at 2677
    Illegal PstI site found at 226
    Illegal PstI site found at 1510
    Illegal PstI site found at 3228
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 214
    Illegal SpeI site found at 2677
    Illegal PstI site found at 226
    Illegal PstI site found at 1510
    Illegal PstI site found at 3228
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1
    Illegal BglII site found at 3265
    Illegal BamHI site found at 1321
    Illegal BamHI site found at 1345
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 214
    Illegal SpeI site found at 2677
    Illegal PstI site found at 226
    Illegal PstI site found at 1510
    Illegal PstI site found at 3228
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 214
    Illegal SpeI site found at 2677
    Illegal PstI site found at 226
    Illegal PstI site found at 1510
    Illegal PstI site found at 3228
    Illegal AgeI site found at 1198
    Illegal AgeI site found at 2518
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 3015
    Illegal SapI.rc site found at 370
    Illegal SapI.rc site found at 418


Design Notes

The restriction sites were added by PCR with designed prymers. Restriction site at 5' Kpn I, and 3' XhoI


Source

This part comes from two Genomic Sequence from the NCBI.

References